Josh Keaton

Peter Parker / Spider Man (voice)

Stan Lee

Stan (voice)

Ed Asner

Ben Parker (voice)

Grey DeLisle

Betty Brant (voice)

Phil LaMarr

Rand Robertson / Robbie Robertson (voice)

Cree Summer

Glory Grant (voice)

Kevin Michael Richardson

Big Man / Tombstone / L. Thompson Lincoln (voice)

Tricia Helfer

Felicia Hardy / Black Cat (voice)

Robert Englund

Vulture (voice)

Crispin Freeman

Electro (voice)

Peter MacNicol

Otto Octavius (voice)

Deborah Strang

May Parker (voice)

Dee Bradley Baker

Curt Connors / Homunculi (voice)

John DiMaggio

Flint Marko / Sandman / Hammerhead (voice)

Ben Diskin

Eddie Brock (voice)

Clancy Brown

Alex O'Hirn / Ox (voice)

Joshua LeBar

Flash Thompson / Car Thief #1 (voice)

Vanessa Marshall

Mary Jane Watson / Rosie Thompson (voice)

Daran Norris

J. Jonah Jameson / John Jameson (voice)

James Arnold Taylor

Patch / Homunculi (voice)

Alan Rachins

Norman Osborn (voice)

Alanna Ubach

Liz Allan / Helena (voice)

Lacey Chabert

Gwen Stacy (voice)

Miguel Ferrer

Silvermane (voice)

Danny Trejo

Ox (voice)

Eric Vesbit

Sergei Kravinoff / Kraven the Hunter (voice)

Dorian Harewood

Doc Bromwell (voice)

Steve Blum

Dilbert Trilby / Seymour O'Reily / Green Gobilin (voice)