Samuel L. Jackson

Afro Samurai / Ninja Ninja (voice)

Phil LaMarr

Brother 1 / Brother 3 / Brother 5 / Kuro / Teen Afro Samurai (voice)

Kelly Hu

Okiku (voice)

Yuri Lowenthal

Kuma / Jinno (voice)

Ron Perlman

Justice (voice)

Grey DeLisle

Oyuki (voice)

John DiMaggio

Brother 2 (voice)

Terrence 'T.C.' Carson

Sword Master (voice)

Fred Tatasciore

Juzo (voice)

James Arnold Taylor

Yashichi (voice)

Jeff Bennett

Foo (voice)

Tara Strong

Otsuru (voice)

Steve Blum

Assassin (voice)

S. Scott Bullock

Dharman (voice)