Tobey Maguire

Spider-Man / Peter Parker

Kirsten Dunst

Mary Jane Watson

James Franco

Harry Osborn

Alfred Molina

Doc Ock / Otto Octavius

Rosemary Harris

May Parker

J.K. Simmons

J. Jonah Jameson

Donna Murphy

Rosalie Octavius

Daniel Gillies

John Jameson

Dylan Baker

Dr. Curt Connors

Bill Nunn

Joseph 'Robbie' Robertson

Vanessa Ferlito

Louise

Aasif Mandvi

Mr. Aziz

Willem Dafoe

Green Goblin / Norman Osborn

Cliff Robertson

Ben Parker

Ted Raimi

Hoffman

Elizabeth Banks

Miss Brant

Bruce Campbell

Snooty Usher

Gregg Edelman

Dr. Davis

Elya Baskin

Mr. Ditkovitch

Mageina Tovah

Ursula

Daniel Dae Kim

Raymond

Hal Sparks

Elevator Passenger

Joel McHale

Mr. Jacks

Stan Lee

Man Dodging Debris

Kelly Connell

Dr. Isaacs

Brent Briscoe

Garbage Man

Emily Deschanel

Receptionist

Jason Fiore-Ortiz

Henry Jackson

Scott Spiegel

Man on Balcony

Andy Bale

OsCorp Executive

Christine Estabrook

Mrs. Jameson

Molly Cheek

Society Woman

John Paxton

Houseman

Joy Bryant

Woman at Web

Joanne Baron

Skeptical Scientist

Peter McRobbie

OsCorp Representative

Timothy Jerome

Injured Scientist

Taylor Gilbert

Mrs. Watson

Peter Vouras

Stage Manager

Donnell Rawlings

Pizza "Heist" Witness

Louis Lombardi

Poker Player

Marc John Jefferies

Amazed Kid

Roshon Fegan

Amazed Kid

Brendan Patrick Connor

Theater Traffic Cop

Reed Diamond

'Algernon'

Dan Callahan

'Jack'

Elyse Dinh

Violinist

John Landis

Doctor

Tim Storms

Chainsaw Doctor

Susie Park

Clawing Nurse

Patricia M. Peters

Screaming Nurse

Michael Edward Thomas

Man at Fire

Anne Betancourt

Woman at Fire

Bill E. Rogers

Fireman

Tom Carey

Train Conductor

Peter Allas

Train Passenger

Brianna Brown

Train Passenger

Bill Calvert

Train Passenger

Tony Campisi

Train Passenger

Joey Diaz

Train Passenger

Chloe Dykstra

Train Passenger

Dan Hicks

Train Passenger

Julia Max

Train Passenger

Timothy Patrick Quill

Train Passenger

Jill Sayre

Train Passenger

Cindy Cheung

Chinese Daughter (uncredited)

Phil LaMarr

Train Passenger (uncredited)

Troy Metcalf

Blue Collar Guy (uncredited)

Bonnie Somerville

Screaming Woman (uncredited)

Garrett Warren

Bearded Doctor (uncredited)

Joseph M. Caracciolo

Priest at Mary Jane's Wedding (uncredited)

Sho Brown

Passenger (uncredited)

Grant Curtis

Hot Dog Vendor (uncredited)

Sam Raimi

University Student with Bag (uncredited)

Denney Pierce

Train Passenger (uncredited)

John Cameron

Man Buying 'Spider-Man No More' Newspaper (uncredited)