Milla Jovovich

Alice

Michelle Rodriguez

Rain

Eric Mabius

Matt

James Purefoy

Spence

Martin Crewes

Kaplan

Colin Salmon

One

Pasquale Aleardi

J.D.

Heike Makatsch

Lisa

Ryan McCluskey

Mr. Grey

Oscar Pearce

Mr. Red

Indra Ové

Ms. Black

Anna Bolt

Dr. Green

Joseph May

Dr. Blue

Robert Tannion

Dr. Brown

Jaymes Butler

Clarence

Stephen Billington

Mr. White

Fiona Glascott

Ms. Gold

Liz May Brice

Medic

Torsten Jerabek

Commando 1

Marc Logan-Black

Commando 2

Michaela Dicker

Red Queen

Jason Isaacs

Dr. William Birkin (uncredited)