James Stewart

Paul "Pauly" Biegler

Lee Remick

Laura Manion

Ben Gazzara

Lieutenant Frederick "Manny" Manion

Arthur O'Connell

Parnell Emmett "Parn" McCarthy

Eve Arden

Maida Rutledge

Kathryn Grant

Mary Pilant

George C. Scott

AAG Claude Dancer

Orson Bean

Dr. Matthew Smith

Russ Brown

Deputy Sheriff George Lemon

Murray Hamilton

Alphonse "Al" Paquette

Brooks West

DA Mitchell "Mitch" Lodwick

Ken Lynch

Det. Sgt. James "Jim" Durgo

John Qualen

Deputy Sheriff Sulo Kangas

Howard McNear

Dr. Adelord Dompierre

Alexander Campbell

Dr. W. Gregory Harcourt

Ned Wever

Dr. Homer Raschid

Jimmy Conlin

Clarence "One-Shot" "Smoky" Madigan

Joseph N. Welch

Judge Harlan Weaver

Royal Beal

Sheriff Max Battisfore

Joseph Kearns

Lloyd Burke

Don Ross

Duane "Duke" Miller

Lloyd Le Vasseur

Court clerk

James Waters

Army Sergeant 1st Class

Duke Ellington

Pie Eye (uncredited)

Irv Kupcinet

Distinguished Gentleman (uncredited)

Chuck Ramsay

Orderly (uncredited)

Agnes Rodgers Brown

Juror (uncredited)